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Micro SSP
micro SSP ® HLA Typing Kits:
Sequence Specific Primers Typing is a PCR based technique which uses Sequence Specific Primers for DNA based Tissue Typing.
One Lambda’s portfolio of Micro SSP typing trays provide complete HLA Class I and Class II DNA typing. Using Generic Trays, technologists can either assign HLA Class I or Class II serological typing individually or assign both simultaneously. If more detailed typing is needed, the High Resolution and Allele Specific Trays will help eliminate redundancies and produce more specific results.
The library of Allele Specific Trays has been optimized to broaden typing coverage and reduce ambiguities. Regular nomenclature updates are used to design new lots in order to resolve new ambiguities. Offering a complementary line of typing products, from LABType™ to Micro SSP to SeCore™, One Lambda ensures that labs worldwide can achieve a wide range of allele coverage. By combining analyses of One Lambda molecular assays in HLA Fusion, users are able to arrive at a high resolution final HLA typing result.
Benefits:
- High degrees of resolution
- Simple post-amplification analysis
- Cost-effective alternative to other molecular methods
- Low and high resolution typing with one method
- Frequently updated for new alleles
- Superior technical and customer service
AllSet +™ Gold SSP Kits: these kits direct to high-resolution for allele-level typing in a single tray.Each Sequence Specific Primers kit consists of a panel of primer mixes that each contain a specific primer pair ( i.e., the allele- and the group-specific primers) as well as a control primer pair that matches non-allelic sequences present in all samples. This control acts as an internal PCR control to verify the efficiency of the PCR amplification.
• A wide selection customized for your typing needs - a variety of kits allow you to select just what you need:
- High-resolution for HLA class I and class II loci
- Low-resolution for HLA class I and class II loci, including combination plates for ABC, ABDR, ABDRDQ, and DRDQ
• Greater accuracy—allele kits result in fewer ambiguities compared to serology or some sequence oligonucleotide (SSO) methods
• Short turnaround time—HLA typing results from DNA isolation in as little as 2 h ours
• Simple to use—one experimental protocol with one plate orientation and a single set of PCR conditions makes training, adoption, and transitioning from one kit to the next easier and faster
• Results you can trust—each test has a color-coded negative control to minimize technician variation and the chance for human error
• Efficient workflow—kits include dried, predispensed primer mixes and prealiquoted buffer, which makes handling easier and faster
• Ready to use—kits are stored at 4°C, so there is no defrosting time
• Less waste—dry formatted kits allow shipments at ambient temperature, which means less packaging and less waste
• Confidence—kits are for in vitro diagnostic use